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Because we were interested in the sequence preference of Glan Dicer-like queenx in an unbiased way, we tested classifeids cleavage activity with a random hairpin RNA substrate.

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Thus, we characterize three Dicer-like enzymes that are involved in Paramecium development and propose a biological role for their sequence-biased cleavage products. In Parameciumeight genes encode for Dicer or Dicer-like enzymes. These Dicer-like enzymes have similar protein domain architecture to the Dicer of Giardia intestinalis.

A Four Dicer-like proteins from Paramecium tetraurelia are shown schematically.

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It is important for the small RNAs to target the precise excision of those DNA elements by helping the excision machinery. Here, we show that Paramecium Dicer-like proteins have sequence specificity for their target dsRNA, which contributes to the efficiency and precision of the DNA elimination machinery. Both developmental classes of small RNAs found in Paramecium contain end signature motifs. ❶But outside, on Saturday July 11th, hot Brazilian music heated up the numerous visitors.

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